Réduction et contrôle du foisonnement filamenteux des boues activées par application d'un régime turbulent

Le foisonnement filamenteux des boues activées est un problème important dans les stations d'épuration du type Boues Activées, car il peut entraîner des pertes de biomasse avec l'eau épurée. Les techniques de lutte utilisées actuellement sont, soit des techniques dites « biologiques » (sélecteurs), soit des techniques chimiques (floculants, biocides). L'objectif de notre travail a été la mise au point d'un procédé original basé sur la création d'une zone de turbulence pour casser les réseaux filamenteux. La réduction et le contrôle du bulking sont obtenus par passage (de durée limitée T1) de la biomasse dans un système venturi, équipée d'une prise d'air. L'efficacité du procédé dépend principalement de la turbulence due à l'auto-aspiration d'air au niveau du rétrécissement du venturi. Cette turbulence contribue d'une part au mécanisme de « cisaillement » des filaments, et d'autre part à la qualité de l'effluent sortant. Une gestion adaptée (aération séquentielle en procédé à faible charge) permet d'obtenir, outre une amélioration de la décantation, une amélioration de l'élimination de l'azote. De plus, le contrôle de la biomasse optimise l'élimination de la pollution carbonée.The loss of biomass in a waste water plant as a result of sludge bulking is an important problem. Biological (ex.: selector configuration) or chemical (ex. : floculating reagents, biocides...) methods habe been used in order to control bulking. A new process based on the generation of turbulence to break the filaments is described. To reduce and control sludge bulking, the biomass is pumped through a venturi equipped with an air inlet. Figure 1 represents the pilot scheme. Two processes can be used : the emulsor with air is introduced on recycling of sludge or is placed in an independent loop. Here, it is the latter that has been used.We tried to reduce bulking by mechanical treatment. The hydrodynamic efficiency of the process depends mostly on the degree of turbulence generated by the waters speed and by the suction of air in the constricted part of the venturi.During the first trials on the plant, we defined the maximum turbulence allowed in order not to break the floc. We could not use a big aeration to prevent defloculation.The main factors to reduce bulking are :- the sequences of usage of the emulsor venturi (the periodicity of the treatment is T),- the speed of the water in the nozzle of the venturi (we can refer to the Reynolds Number Re = Ud/ʋ; but this number can’t really represent the turbulence created),- the duration of the emulsor's application. The emulsor venturi is used during a period T1 with a periodicity T (T>T1), with T1 : n X (VR/qv) where VR/qv is the contact time in the venturi and n is the frequence of recycling.During the period T, it is possible to use continuous aeration or to work with sequential aeration.a) continuous aeration|_________________________| TTime between two applications of the venturi|_______________| T1Venturi durationThe process is the same as the aeration of activated sludge. COD is reduced but only with low load, significant nitrification occurs. Figure 5 shows the efficiency of the continuous aeration process especially versus the sludge index parameter.b) sequential aerationAs we try to remove nitrogen, the sequential aeration is more useful than continuous aeration. A cycle is divided in two periods (T2, T3 with T = T2 + T3), and of course, the duration of the venturi use equal to T1.|_________________________| TTime between two applications of the venturi|_____________| T1Venturi duration|_________________| T2Aeration duration          |_____| T3Anoxic periodFigures 6 and 7 show the efficiency of the emulsor if we consider lB (sludge index) as the main parameter for the sequential operation (T2, T3) and it's also possible to obtain a good removal of nitrogen pollution.Our operations parameters were for these experiments :T = 8 or 12 hoursT1 between 20 min. and 1 hourT2 between 4 and 5 hoursThis process is now patiented and the first industrial applications are now defined

Fermi-surface transformation across the pseudogap critical point of the cuprate superconductor La1.6−x_{1.6-x}Nd0.4_{0.4}Srx_{x}CuO4_4

The electrical resistivity $\rho$ and Hall coefficient R$_H$ of the tetragonal single-layer cuprate Nd-LSCO were measured in magnetic fields up to $H = 37.5$ T, large enough to access the normal state at $T \to 0$, for closely spaced dopings $p$ across the pseudogap critical point at $p^\star = 0.235$. Below $p^\star$, both coefficients exhibit an upturn at low temperature, which gets more pronounced with decreasing $p$. Taken together, these upturns show that the normal-state carrier density $n$ at $T = 0$ drops upon entering the pseudogap phase. Quantitatively, it goes from $n = 1 + p$ at $p = 0.24$ to $n = p$ at $p = 0.20$. By contrast, the mobility does not change appreciably, as revealed by the magneto-resistance. The transition has a width in doping and some internal structure, whereby R$_H$ responds more slowly than $\rho$ to the opening of the pseudogap. We attribute this difference to a Fermi surface that supports both hole-like and electron-like carriers in the interval $0.2 < p < p^\star$, with compensating contributions to R$_H$. Our data are in excellent agreement with recent high-field data on YBCO and LSCO. The quantitative consistency across three different cuprates shows that a drop in carrier density from $1 + p$ to $p$ is a universal signature of the pseudogap transition at $T=0$. We discuss the implication of these findings for the nature of the pseudogap phase.Comment: 11 pages, 12 figure

Optimizing and developing a scalable, chemically defined, animal component-free lentiviral vector production process in a fixed-bed bioreactor

Lentiviral vectors (LVVs) play a critical role in gene delivery for ex vivo gene-modified cell therapies. However, the lack of scalable LVV production methods and the high cost associated with them may limit their use. In this work, we demonstrate the optimization and development of a scalable, chemically defined, animal component-free LVV production process using adherent human embryonic kidney 293T cells in a fixed-bed bioreactor. The initial studies focused on the optimization of the culture process in 2D static cultures. Process changes such as decreasing cell seeding density on day 0 from 2.5 × 104 to 5 × 103 cells/cm2, delaying the transient transfection from 24 to 120 h post-seeding, reducing plasmid DNA to 167 ng/cm2, and adding 5 mM sodium butyrate 6 h post-transfection improved functional LVV titers by 26.9-fold. The optimized animal component-free production process was then transferred to the iCELLis Nano bioreactor, a fixed-bed bioreactor, where titers of 1.2 × 106 TU/cm2 were achieved when it was operated in perfusion. In this work, comparable functional LVV titers were obtained with FreeStyle 293 Expression medium and the conventional Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum both at small and large scale

Pseudogap phase of cuprate superconductors confined by Fermi surface topology

The properties of cuprate high-temperature superconductors are largely shaped by competing phases whose nature is often a mystery. Chiefly among them is the pseudogap phase, which sets in at a doping $p^*$ that is material-dependent. What determines $p^*$ is currently an open question. Here we show that the pseudogap cannot open on an electron-like Fermi surface, and can only exist below the doping $p_{FS}$ at which the large Fermi surface goes from hole-like to electron-like, so that $p^*$ $\leq$ $p_{FS}$. We derive this result from high-magnetic-field transport measurements in La$_{1.6-x}$Nd$_{0.4}$Sr$_x$CuO$_4$ under pressure, which reveal a large and unexpected shift of $p^*$ with pressure, driven by a corresponding shift in $p_{FS}$. This necessary condition for pseudogap formation, imposed by details of the Fermi surface, is a strong constraint for theories of the pseudogap phase. Our finding that $p^*$ can be tuned with a modest pressure opens a new route for experimental studies of the pseudogap.Comment: 15 pages, 5 figures, 7 supplemental figure

Protection against Clostridium difficile infection in a hamster model by oral vaccination using flagellin FliC-loaded pectin beads

International audienceClostridium difficile flagellin FliC is a highly immunogenic pathogen-associated molecular pattern playing a key role in C. difficile pathogenesis and gut colonization. Here, we designed an oral vaccine against C. difficile with FliC encapsulated into pectin beads for colonic release. Bead stability and FliC retention was confirmed in vitro using simulated intestinal media (SIM), while bead degradation and FliC release was observed upon incubation in simulated colonic media (SCM). The importance of FliC encapsulation into pectin beads for protection against C. difficile was assessed in a vaccination assay using a lethal ham-ster model of C. difficile infection. Three groups of hamsters orally received either FliC-loaded beads or unloaded beads in gastro-resistant capsule to limit gastric degradation or free FliC. Two other groups were immunized with free FliC, one intra-rectally and the other intra-peritoneally. Hamsters were then challenged with a lethal dose of C. difficile VPI 10463. Fifty percent of hamsters orally immunized with FliC-loaded beads survived whereas all hamsters orally immunized with free FliC died within 7 days post challenge. No significant protection was observed in the other groups. Only intra-peritoneally immunized hamsters presented anti-FliC IgG antibodies in sera after immunizations. These results suggest that an oral immunization with FliC-loaded beads probably induced a mucosal immune response, therefore providing a protective effect. This study confirms the importance of FliC encapsulation into pectin beads for a protective oral vaccine against C. difficile

Radiotherapy in breast cancer: current standards of treatment, prediction of local recurrence and open questions

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Automatic Calibration of Artificial Neural Networks for Zebrafish Collective Behaviours using a Quality Diversity Algorithm

During the last two decades, various models have been proposed for fish collective motion. These models are mainly developed to decipher the biological mechanisms of social interaction between animals. They consider very simple homogeneous unbounded environments and it is not clear that they can simulate accurately the collective trajectories. Moreover when the models are more accurate, the question of their scalability to either larger groups or more elaborate environments remains open. This study deals with learning how to simulate realistic collective motion of collective of zebrafish, using real-world tracking data. The objective is to devise an agent-based model that can be implemented on an artificial robotic fish that can blend into a collective of real fish. We present a novel approach that uses Quality Diversity algorithms, a class of algorithms that emphasise exploration over pure optimisation. In particular, we use CVT-MAP-Elites, a variant of the state-of-the-art MAP-Elites algorithm for high dimensional search space. Results show that Quality Diversity algorithms not only outperform classic evolutionary reinforcement learning methods at the macroscopic level (i.e. group behaviour), but are also able to generate more realistic biomimetic behaviours at the microscopic level (i.e. individual behaviour).Comment: 8 pages, 4 figures, 1 tabl